Description
- Fast protocol: 10 min of hands-on time, 1.5 hrs of protocol time
- Less magnetic beads required for cleanup: Save the cost more than 50%
ChIP-Seq Library Prep Kit (illumina and MGI Platforms)
The ChIP-Seq Library Prep Kit (illumina and MGI Platform) was developed for the construction of high quality ChIP-Seq libraries using 5 ng to 400 ng of ChIP DNA as input. The kit is compatible with ChIP DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.).
ChIP-Seq Library Prep Kit Workflow
ChIP-Seq is the combination of chromatin immunoprecipitation (ChIP) with next generation sequencing. It is a powerful tool for the analysis of global transcription factors and other proteins in diseases and biological pathways, and characterization of histone modifications in a genome-wide level at single-base resolution. ChIP-Seq delivers whole genome level of functional profiling of global transcription factors, and provides better understanding of epigenetic modifications.
Comparison of library conversion efficiency under the same condition. Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30036S) was used.
Library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. The unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34034).
Data comparison: Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30036S) was used. Sequencing peak regions are shown.
Sample | Total Reads | Aligned Reads | Aligned Rate | Duplication Rate |
Control | 15711531 | 12845306 | 81.8% | 3.6% |
5 ng input | 10589190 | 8958002 | 84.6% | 3.2% |
30 ng input | 15350625 | 12520807 | 81.6% | 3.4% |
Comparison of aligned reads, aligned rate and duplication rate. Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30036S) was used.
Features
- Fast protocol: 10 min of hands-on time, 1.5 hrs of protocol time
- Less magnetic beads required for cleanup steps: Save the cost more than 50%
- Input ChIP DNA: From 5 ng to 400 ng