Description
Enzyme-based fragmentation of DNA is very simple and effective comparing to the mechanical DNA shearing methods. There are several advantages of enzymatic DNA fragmentation, include easy handling of many samples at the same time, and less loss of samples. Moreover, it does not require expensive capital expense for the equipment.
DNA Fragmentation Enzyme Workflow
The resulting DNA fragment size is inversely correlated with the incubation time of step 1 at 20°C. The fragmented DNA can be used for applications such as Next-Generation Sequencing (NGS). Our Fragmentation Enzyme does not generate detectable sequencing bias. Sequence coverage is also consistent between enzymatic and mechanical fragmentation.
DNA fragment size is inversely correlated with the incubation time of step 1 at 20°C.
DNA Fragmentation & A-tailing Enzyme Mix (Cat. # 40062)
The enzyme mix can be used for enzymatic fragmentation of genomic DNA and addition of A-tailing at 3’-ends of DNA in a single step. 3’-ends of DNA with A-tailing can be used in many downstream applications in the field of molecular biology such as next generation sequencing, PCR cloning, and TA-ligation etc.
Features
- Fast enzymatic fragmentation: 30-45 minutes
- Simple Procedure: 3 minutes of hands-on time
- Works with both EDTA-free DNA and DNA resuspended in TE buffer
- Ideal for downstream reactions: NGS library prep, PCR cloning, TA-ligation, and blunt end ligation etc.
