dsDNA Quantification Broad Range Kit (for Qubit®)

Description

The dsDNA Quantification Broad Range Kit is developed for double stranded DNA quantification. The DNA Quantification BR kit includes BR Dye, BR Dilution Buffer, and two DNA Standards. Simply dilute the BR Dye with the BR Dilution Buffer to make 1X working solution, add DNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for DNA concentrations from 0.2 ng/µL to 2000 ng/µL with a detection range of 4 ng – 2000 ng, and is highly selective for double-stranded DNA over RNA.

The 1X working solution can be stored at 4°C and protected from light, stable for up to 2 months.

Features

• Kit was optimized for use with the Qubit Fluorometer
• Uses the Qubit dsDNA Broad Range assay setting
• Detection range is 4-2000 ng dsDNA
• 1X working solution (mixture of dye and dilution buffer) can be stored at 4°C and protected from light, stable for up to 2 months.
• Great saving since the kit cuts the costs by 67%

DNA selectivity and sensitivity. A series of input DNA (200, 400, 600, 800, and 1000 ng) was used with or without RNA contamination.

The BioDynami dsDNA BR kit is nearly identical to that of Thermo Fisher’s Qubit dsDNA BR kit.

Common contaminants such as salts, free nucleotides, solvents, detergents, RNA, single stranded DNA, or protein are well tolerated in the assays.