Description
The dsDNA Quantification High Sensitivity Kit is developed for double stranded DNA quantification. The DNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two DNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer to make 1X working solution, add DNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for DNA concentrations from 0.005 ng/µL to 120 ng/µL with a detection range of 0.1 ng – 120 ng, and is highly selective for double-stranded DNA over RNA.
The 1X working solution can be stored at 4°C and protected from light, stable for up to 5 days.
Features
- Optimized condition for using with the Qubit Fluorometer
- Uses the Qubit dsDNA High Sensitivity assay setting
- Linear range: 0.1 ng – 120 ng dsDNA
- 1X working solution (mixture of dye and dilution buffer) can be stored at 4°C and protected from light, stable for up to 5 days.
- Cuts costs by 67%
DNA selectivity and sensitivity of the HS kit. A series of input DNA (20, 40, 60, 80, 100, and 120 ng) was used with or without the contamination of RNA.
The BioDynami dsDNA HS kit is nearly identical to that of Thermo Fisher’s Qubit dsDNA HS kit.
Contaminant | Final concentration in the assay | Concentration in 10-μL sample | Result |
Sodium chloride | 10 mM | 200 mM | OK |
Magnesium chloride | 5 mM | 100 mM | OK |
Sodium acetate | 10 mM | 200 mM | OK |
Ethanol | 1% | 20% | OK |
Phenol | 0.1% | 2% | OK |
SDS | 0.01% | 0.2% | OK |
Triton X-100 | 0.003% | 0.05% | OK |
dNTP | 100 μM | 2 mM | OK |
BSA | 1 mg/mL | 20 mg/mL | OK |
RNA | 0.5X | 0.5X | OK |
ssDNA | 0.5X | 0.5X | OK |
Common contaminants such as salts, free nucleotides, solvents, detergents, RNA, single stranded DNA, or protein are well tolerated in the assays.