ELISABright Chemiluminescent substrate

ELISABright is an enhanced and highly sensitive chemiluminescent HRP substrate optimized for chemiluminescent ELISA applications. This luminol-based substrate demonstrates a wide linear dynamic range (Figure 1) and a superior signal to noise ratio (Figure 2) for excellent quantitative ability. ELISABright allows for enhanced detection of low abundance proteins and reduced consumption of antibodies and other reagents.

Figure 1. ELISABright has a broad linear dynamic range. C3 protein was detected using a sandwich-style ELISA. Plates were coated with anti-C3 antibody and blocked with 1% BSA-PBST. 50 microliters of a series of C3 solutions, ranging in concentration from 7.8 to 500 pM, was added to each well, and C3 protein was detected with a monoclonal anti-huC3a antibody followed by a goat anti-mouse HRP conjugate. Detection was performed according to the ELISABright protocol, using 100 microliters of substrate per well.

Figure 2. ELISABright has a superior signal-to-noise ratio. ELISABright, SuperSignal® ELISA Pico and SuperSignal ELISA Femto substrates (Thermo Fisher) were used to develop replicate sandwich-style ELISA assays as described in Figure 1. Signal-to-noise ratios for detection of wells containing 50 microliters of a 500 pM solution of C3 protein are shown. ELISABright demonstrates a signal-to-noise ratio 6-times higher than the competition.