Description
The kits (listed below) were developed to capture the MHC locus from whole genome NGS libraries based on the CATCH-Seq technology. Moreover, kits combining NGS library prep and MHC locus capture are also available. The integration of library prep and MHC capture not only simplifies the procedure, but also enhances the capture efficiency. Comprehensive sequencing of the selected locus with our kit make it possible to detect SNPs, indels, and structural variants that are not covered by other MHC targeted sequencing reagents.
Our MHC kits enable the most cost-effective targeted sequencing of the human MHC locus, including all coding and non-coding regions.
Features:
- Integration of library prep with capture or capture alone
- Simplifies the procedure
- Enhances the capture efficiency
- Multiple choices of targeted MHC regions
- Kits available for the following regions
- Full 3.8 MB of MHC region
- 2.1 MB Class I region
- 0.7 MB Class II region
- 1.1 MB Class III region
- 190 kb MHC core regions (22 MHC genes)
- Covers exons, introns, 5’ regulatory regions, 3’ regulatory regions, and beyond.
- Kits available for the following regions
- Easy detection of SNPs, indels, and structural variants
- The only reagent provides intact sequence information
- Low cost
- Sample multiplexing: up to 96 samples can be pooled together to further reduces the cost
- Integration of library prep with capture or capture alone
List of MHC Targeted Sequencing Kits
Two types of indexes are available:
Index: For kits with 24 reactions. Sample multiplexing up to 24 libraries is possible. Each of the index primers (#1~#24) has a unique 6-base index sequence that can be used to identify libraries. Details about the index can be found here.
Unique dual index: For kits with 96 and 384 reactions. Sample multiplexing up to 96 libraries is possible. Details about the unique dual index can be found here.