Description

NGS DNA Library Prep Kit (Ion Torrent Platform)

The NGS DNA Library Prep Kit (Ion Torrent platform) was developed for construction of high quality DNA libraries for next generation sequencing using Ion Torrent platform. The kit uses short double strand DNA fragments (blunt ends and/or sticky ends) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA Fragmentation Enzyme Mix and DNA Fragmentation & A-tailing Enzyme Mix etc) and mechanical methods (sonication, nebulization etc.). Our unique technology increases library conversion efficiency and eliminates insert concatemer ligation. Library multiplexing up to 12 samples is possible.

NGS DNA Library Prep Kit Workflow

Features

• Simple procedure: Reactions for all three steps are in one tube
• Fast protocol
  • Total protocol time is around 1 hour
  • Hands-on time is only ~5 minutes
• Input DNA amount: from 50 ng to 1 ug
• No insert concatemer ligation

Comparison of library conversion efficiency

Concatemer ligations are not present in BioDynami kit