Description
Proteinase K is a serine protease that exhibits broad protein cleavage activity. It is well-used for protein removal, PCR purification, nucleic acid extraction such as genomic DNA, RNA, and plasmid.
Proteinase K has higher proteolytic activity in the presence of SDS and urea. The recombinant proteinase K has high activity and works in a wide range of pH and temperature. The recommended Proteinase K working concentration is 50–100 μg/ml.
Features
- Ready-to-use solution format
- Optimal activity between 20 and 60°C. High activity between 50 to 55°C.
- Optimal activity in a pH range of 4.5-12.0; High activity in the pH range of 7.5-11.5.
- No detectable DNase, RNase, endonuclease, and exonuclease activities.
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Applications
- Inactivation of DNases, RNases and other enzymes in reactions
- Removal of DNases and RNases for extraction of genomic DNA and RNA from tissues or cells
- Plasmid DNA extraction
- Enhancement of PCR fragment cloning efficiency
- PCR purification