Description
Long oligos (>50 nt) and ssDNA can be quantified with ssDNA Quantification kits using a Qubit Fluorometer. However, it is difficult to quantify the concentration of oligos that are shorter than 50 nt due to the inaccuracy of the data. To address the issue, we have developed the Short Oligo Quantification Kit for the quantification of oligos that are shorter than 50 nt using Qubit Fluorometer. The kit can quantify the concentration of oligos as short as 11 nt.
The kit includes Short Oligo Dye, Short Oligo Dilution Buffer, and two Standards. Simply dilute the Dye with the Dilution Buffer, add oligo sample (volume from 1 to 20 μL), then read the oligo concentration using the Qubit® Fluorometer. The assay is accurate for oligo concentrations from50 pg/µL to 200 ng/µL based on the line corresponding of the data to standards.
Features
- Quantify the concentration of oligos from 11 to 50 nt
- Use the Qubit® ssDNA assay setting
- Linear range: 1-200 ng oligo
Common contaminants such as salts, solvents, or detergents are well tolerated in the assay (Table 1).
