Description
Features
- Optimized for use with the Qubit Fluorometer
- Uses the Qubit ssDNA assay setting
- Linear range: 1-200 ng ssDNA
- Cost saving
The ssDNA Quantification Kit is developed for single stranded DNA quantification. The kit includes ssDNA Dye, ssDNA Dilution Buffer, and two ssDNA Standards. Simply dilute the ssDNA Dye with the ssDNA Dilution Buffer, add DNA sample (volume from 1-20 μL), then read the concentration using the Qubit® Fluorometer. The assay is accurate for DNA concentrations from 50 pg/µL to 200 ng/µL based on the line corresponding of the data to standards.
Our kit detects ssDNA by using fluorescent dye that enables sensitive single stranded DNA quantification , including ssDNA viruses, synthetic ssDNA, first-strand cDNA synthesis, denatured DNA, and bisulfate-converted DNA etc. ssDNA quantification is essential for the study of the biological process involves ssDNA.
We have developed the Qubit assays with the volume of 100 µL. The volume is half of the standard 200 µL assay, and the reagent costs are reduced to half of the 200 µL assay. The ssDNA kits can save 67% (200 µL assay) and 83% (half-volume 100 µL assay) of the costs as compared to ThermoFisher’s Qubit ssDNA kits.
A series of input ssDNA (200, 400, 600, 800, 1000, and 1200 ng) was used.
The performance of the BioDynami ssDNA Quantification Kit is nearly identical to that of Thermo Fisher’s Qubit ssDNA kit (figure below).
Comparison of BioDynami ssDNA Kit with Thermo Fisher kit.
Common contaminants such as salts, solvents, or detergents are well tolerated in the assay (Table 1).
Contaminants has been tested in BioDynami ssDNA Kit.