Description
- High sensitivity – Detect very low amounts of protein down to femtogram levels
- Ultra-low background – Faint bands are clearly seen without high general background
- Long-lasting signal – Capture multiple exposures without worrying about signal decay
- Linear signal over broad dynamic range – Low and high abundance proteins can be imaged simultaneously with a range spanning over three orders of magnitude
Description
WesternBright Quantum HRP substrate
WesternBright® Quantum® chemiluminescent substrate sets the bar for sensitivity and quantitative ability. Specially developed for CCD imaging, WesternBright Quantum produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Since it does not exhibit substrate depletion at high protein loads, WesternBright Quantum provides the largest dynamic range of any chemiluminescent substrate for the most quantitative chemiluminescent Western experiments.
WesternBright Quantum exhibits high sensitivity with the greatest linear range
WesternBright Quantum produces the most stable chemiluminescent signal
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Blots detected using WesternBright Quantum or one of three other chemiluminescent substrates were re-imaged at several times over a 10 hr period. The intensitiy of one band is plotted. 60 min after substrate incubation, WesternBright Quantum retains 70% of its initial signal strength, while the competition decays to 5% or less.
Extremely low background when using WesternBright Quantum
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Replicate Western blots were developed using WesternBright Quantum or one of three other chemiluminescent substrates. After a simultaneous 20 min exposure to the same piece of film, WesternBright Quantum displays the best combination of sensitivity and low background signal. All display parameters were identical across all images shown in this figure.
High sensitivity for quantitative detection of low-abundance proteins
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WesternBright Quantum provides the highest sensitivity and broadest linear dynamic range. Serial dilutions of A431 cell lysates were blotted and probed to detect STAT-1 protein. The blots were detected with WesternBright Quantum,or two other chemiluminescent substrates (ECL and ECL Plus, GE Healthcare). Only data points on the linear portion of the best-fit curve for each substrate shown. All display parameters were identical across all images in this figure.
No need to rush to image your Western blot
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WesternBright Quantum allows blots to be imaged several hours after substrate incubation. Blots can be re-imaged to obtain the perfect exposure, without worrying about losing signal. A blot was imaged with 2 min exposures at 5 min, 60 min, and 10 hours after substrate incubation. The same band is clearly seen in each image, even after 10 hours.
Superior sensitivity with film
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WesternBright Quantum provides high-performance film detection.WesternBright Quantum or SuperSignal® West Pico (Thermo Scientific) were used to detect duplicate Western blots. A band (arrow) is detected by WesternBright Quantum in a brief exposure, while a much longer exposure is needed to detect the same band with SuperSignal West Pico. All display parameters were identical across all images in this figure